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Effect of Wortmannin on Ang II stimulation of PI3-kinase activity in WKY and SHR neurons. Neuronal cultures of WKY rats and SHRs were incubated with 100 nm Ang II for 15 min in the absence and presence of 100 nm Wortmannin. PI3-kinase activity was determined as described in Materials and Methods. A, Representative autoradiogram.B, Mean ± SE (n = 3). * Significantly different from untreated cells (p < 0.01). ** Significantly different from Ang II-treated cells (p < 0.01).
