Fig. 6.
Neuronal cultures of WKY rats and SHRs were incubated with 100 nm Ang II in the absence or presence of 100 nm Wortmannin for 4 hr at 37°C. Levels of NET mRNA (A), TH mRNA (B), and [3H]-NE uptake (C) were measured essentially as described in Materials and Methods. A, B, Top, Representative autoradiograms; bottom, mean data from three experiments ± SE. * Significantly different from control (p < 0.01). ** Significantly different from Ang II-treated neurons (p < 0.05). # Significantly different from Ang II-treated WKY rat neurons (p < 0.01).