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Effect of Wortmannin on Ang II stimulation of AP-1 binding activity in the nuclear fractions of WKY and SHR neurons. Nuclear extracts of control, Ang II-treated (100 nm) or Ang II + Wortmannin-treated WKY rat and SHR neurons were subjected to gel shift analysis to determine the levels of AP-1 binding activity essentially as described in Materials and Methods.
