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. 1999 Apr 1;19(7):2413–2423. doi: 10.1523/JNEUROSCI.19-07-02413.1999

Fig. 9.

Fig. 9.

Effect of Ang II on protein kinase B activity in WKY and SHR neurons. A, Time course: neuronal culture of WKY rat and SHR were incubated with 100 nm Ang II for the indicated time at 37°C. PKB activity in neuronal extract was determined essentially as described in Materials and Methods.Top, Representative autoradiogram;bottom, mean ± SE (n = 3). * Significantly different from zero time (p< 0.05). # Significantly different from the WKY (p < 0.05). B, Ang II concentrations: neuronal cultures of WKY rat and SHR were incubated with the indicated concentrations of Ang II for 10 min at 37°C before determination of PKB activity. Top, Representative autoradiogram; bottom, mean ± SE (n = 3). * Significantly different from control (p < 0.05). # Significantly different from the WKY (p < 0.05). C, Effect of Ang receptor antagonists: experimental conditions were essentially as described in the legend to Figure10B. WKY rat and SHR neuronal cultures were incubated without (C) or with 100 nmAng II in the absence or presence of 10 μm losartan (Los) or PD123319 (PD).Top, Representative autoradiogram;bottom, mean ± SE (n = 3). * Significantly different from control (p< 0.05). ** Significantly different from Ang II treatment (p < 0.05).