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. 1999 Apr 1;19(7):2424–2431. doi: 10.1523/JNEUROSCI.19-07-02424.1999

Fig. 3.

Fig. 3.

Effect of METH on striatal DAT-Nt immunoreactivity. Animals were treated with METH (15 mg/kg, s.c.), and striata were harvested 7 d after the drug administration. Densitometric analysis of DAT-Nt immunoreactivity was performed in both samples from individual animals (7 mice per group) and pooled samples for each group. Data are presented as a representative blot using pooled samples from each group. Immunoreactive bands of 85 kDa for DAT-Nt and 55 kDA for α-tubulin were observed. Lanes 1–5, Standard curve from wild-type mouse striatum (1.25, 2.5, 5, 10, and 20 μg, respectively). Lane 6, Wild-type mice, saline-treated (10 μg). Lane 7, Wild-type mice, METH-treated (10 μg). Lane 8, VMAT2+/− mice, saline-treated (10 μg). Lane 9, VMAT2+/− mice, METH-treated (10 μg). Individual measurements revealed a 32.1 ± 5% reduction in wild-type versus 61 ± 11.4% reduction in the VMAT2 +/− mice after METH.p < 0.05, between genotypes.