Table 3.

Conduction of MGA APs in intact or PEG-fused MGAs versus postoperative time

PO	n	% MGAs	CV(n) ± SD	Conditions
Intact MGAs, sham-operated,in vivo
1 d	16	88	14 (7)  ± 3
5 d	15	87	11 (6)  ± 4
15 d	16	94	15 (6)  ± 3
30 d	20	95	13 (5)  ± 4
Intact MGAs, PEG hydrogel, in vivo
1 d	24	92	11 (7)  ± 4
5 d	24	96	12 (7)  ± 5
15 d	21	81	13 (6)  ± 3
30 d	15	87	14 (5)  ± 4
PEG-fused MGAs, in vitro
0.5–24  hr	31	16
0.5–24  hr	17	47		PEG hydrogel
PEG-fused MGAs, in vivo
1 d	99	27		Anesthetic
1 d	12	25		PEG hydrogel
5–15 d	11	27		PEG hydrogel
20 d	2	50		PEG hydrogel

PO, Postoperative time. n, Total number of MGAs tested. % MGAs, Number of MGAs tested that conducted an AP through the lesion site, site of sham operation, and/or site of hydrogel application, divided by the total number of MGA preparations tested (n), times 100%. CV(n) ± SD, Conduction velocity (m/sec) and SD of number (n) of MGA APs traveling in a rostral-to-caudal direction through the lesion site (APs are normally conducted rostrally-to-caudally in MGAs in the intact animal). PEG hydrogels were not applied, and earthworms were not anesthetized, unless explicitly stated. p > 0.10 (Student’st test) for any paired set of CVs from sham-operated versus hydrogel-treated MGAs.