Figure 7. The effect of MnIII complex on expression of apoptosis- and cell cycle-related genes.
The MCF-7 and MDA-MB-231 cells were either untreated or treated with Mn complex for 24 h. The mRNA expression levels of indicated genes were determined using real-time PCR. The results indicated an increase in Bax/Bcl-2 ratio and up-regulation of TNF-α, caspase-3, −8 and −10. Altered expression of cell cycle-related genes including over expression of p21 along with down-regulation of cyclin D1 were also detected in treated breast cancer cells. The results were expressed as the mean ± SD (n = 3). ∗P < 0.05 was considered statistically significant compared to the control group.