Fig. 4.

AKBA exhibits potent antitumor activity in docetaxel-resistant homograft mice. a The body weights were measured every 2 days after the indicated treatment. b, c The volume of tumors from different groups was recorded every 2 days. Data are represented as the means ± SEM. In a–c, *P < 0.05 and ***P < 0.001 compared with the vehicle control in RM-1/Doc or RM-1 group. d Effect of AKBA on tumor weight was detected at the time of sacrifice. Data are shown as the means ± SEM. e Representative tumors from the six groups are shown. f, g Inhibitory effect of AKBA on tumor cell proliferation was also confirmed with the decreased Ki67-positive cells in RM-1/Doc homografts. Representative images of H&E and Ki67 staining of the indicated treated groups (magnification, 100×) (f). Ki67-positive rates in the two groups (g). Data are shown as the means ± SEM. **P < 0.01 compared with the control. h Western blotting analysis of phosphor-Akt and phosphor-Stat3 expression in RM-1/Doc homografts treated with vehicle or AKBA