Fig. 5.

UNC0642 induces apoptosis in UBC cells. a–c T24, J82, and 5637 cells were treated with UNC0642 at the indicated concentrations for 72 h. Apoptosis was determined with an Annexin V/PI assay (a). Cells in the first and forth quadrants were defined as apoptotic cells. The assays were performed in triplicate, and the results are presented as the mean ± SD (b). Cell lysates were analyzed via western blotting with the indicated antibodies (c). d T24 cells were treated with UNC0642 at the indicated concentrations for 48 h. The expression levels of Ampkα2, ELL2, and Bim were determined with qRT-PCR. *P < 0.05; **P < 0.01; ***P < 0.001