Fig. 8.

Disulfide cross-linking protects AgUricase from protease digestion. Wild-type (circle), K12C–E286C (square), and K12C–E286C-K244C (triangle) mutant AgUricase (80 μg) were treated with 20 μg Proteinase K at room temperature, and the residual activity of AgUricase was measured at various times. The data are presented as the mean ± standard error of the mean (SEM) of at least three independent experiments