Fig. 5.

IMB17–15 significantly activated AMPK pathway and regulated various genes involved in BA and lipid metabolism in hamster liver tissues. a mRNA expressions of Fas, Acc, Ldlr, Srebp1c, Scd1 and Pparα in the control, HFD + NS and HFD + IMB17–15 livers were normalized against Actb. b The protein expression levels of total and phosphorylated P-AMPKα and PPARα in hamster liver tissues, β-actin served as the loading control. ACC acetyl-CoA carboxylase, AMPKα adenosine monophosphate (AMP)-activated protein kinase, FAS fatty acid synthase, LDLR low-density lipoprotein receptor, PPARα peroxisome proliferator activated receptor α, SCD1 stearoyl-CoA desaturase, SREPB1C sterol regulatory element-binding protein 1c. The values are expressed as the mean ± SD (n = 6 in each group), ^#P < 0.05 versus the control group, *P < 0.05 versus the HFD + NS group, **P < 0.01 versus the HFD + NS group; ANOVA followed by Duncan's multiple range tests