Fig. 1.

PRDX6 knockdown through inducible lentiviral knockdown system enhances ferroptotic LOOH. a Western blot analysis of PRDX6 expression in indicated genetically engineered H1299 cells after doxycycline treatment (0.25 μg/mL) for 48 h, b LOOH production was assayed by flow cytometry using C11-BODIPY. c After doxycycline (0.25 μg/mL) treatment for 48 h, indicated genetically engineered H1299 cells were treated with Erastin (2 μM) for 12 h, and LOOH production was assayed. Representative data are shown and the statistical data are from three independent experiments and the bar indicates the mean value (***P < 0.001). d After doxycycline treatment (0.25 μg/mL) for 48 h, indicated genetically engineered H1299 cells were treated with RSL-3 (50, 100, or 150 nM) for 12 h, and LOOH production was assayed. e After doxycycline (0.25 μg/mL) treatment for 48 h, indicated genetically engineered H1299 cells were treated with Erastin (2 μM) with or without ferrostatin-1 (10 μM) for 12 h, and LOOH production was assayed