Fig. 3.

PRDX6 negatively regulates ferroptosis through its iPLA2 activity. a After infection of lentivirus targeting to PRDX6 for 48 h, H1299 cells were treated with Erastin (2 μM) for 6 h and intracellular GSH levels were examined. The statistical analysis is from three independent experiments and the bar indicates the mean values. b, c H1299 cells were treated with Erastin (2 μM) with or without of MJ33(5 μM) or ferrostatin-1 (10 μM) for 12 h, and LOOH production was assayed by flow cytometry using C11-BODIPY. Representative data are shown and the statistical analysis is from three independent experiments and the bar indicates the mean values. d, e After infection for 48 h, H1299 cells were treated with Erastin (2 μM) with or without MJ33 (5 μM) for 12 h, and LOOH production was assayed. Representative data are shown and the statistical analysis is from three independent experiments and the bar indicates the mean values. (*P < 0.05; ***P < 0.001; n.s.: no statistic difference)