Table 6.

Time course of pyrotinib recovery after incubation with human plasma and resuspension with phosphate buffer, pH 7.4

Incubation time (h)	Concentration (µg/mL)	Recovery (%)a
0	20.5	103
6	16.6	83.0

Reconstitution time (h)	Concentration (µg/mL)	Recovery (%)b
0	0.0336	0.988
16	0.715	21.0

^apercentage of extractable pyrotinib accounting for pyrotinib added into human plasma

^bpercentage of pyrotinib recovered after resuspension with phosphate buffer accounting for pyrotinib covalently bound with human plasma