Fig. 4.

The pro-angiogenic effect of irisin was associated with the ERK signaling pathway. a Representative images and quantification of phosphorylated and total ERK protein expression in HUVEC analyzed by Western blotting. The ERK inhibitor U0126 was used to block ERK phosphorylation, and β-actin was used as the internal control. n = 5. b Representative images and quantification of phosphorylated and total ERK protein expression in MI mice analyzed by Western blotting. n = 5. c Representative images and quantification of HUVEC migration detected by transwell migration assay. n = 3, scale bar = 100 μm. d Representative images and quantification of HUVEC migration detected by scratch wound-healing assay. n = 3, scale bar = 100 μm. *P < 0.05 vs. control