Fig. 3.

Propofol, via repression of the expression of MMP-9, could attenuate the effect of TNF-α on the integrity of the in vitro BBB model. a Evaluation of the in vitro BBB model integrity by measuring TEER over the course of 7 days coculturing of hCMEC/D3 cells and human astrocytes. The panel is the value of TEER that measured every day over the course of 7 days. Data were repressed as the mean ± SD. *P < 0.05, compared with Day 1. Each assay was performed in five replicates. b Evaluation of TEER in the in vitro BBB model after treatment. Data are expressed as the mean ± SD. *P < 0.05, compared with the control group. ^#P < 0.05, compared with the TNF-α treatment group. Each assay was performed in five replicates. c Evaluation of TEER in the in vitro BBB model that was measured after each treatment. Please note that the control group was treated with siRNA-NC. Data are expressed as the mean ± SD. *P < 0.05, compared with the control group. ^#P < 0.05, compared with the TNF-α treatment group. Each assay was performed in five replicates. d Evaluation of TEER in an in vitro BBB model that was measured after each treatment. Data are expressed as the mean ± SD. Please note that the control group was treated with siRNA-NC. *P < 0.05, compared with the control group. Each assay was performed in five replicates