Fig. 3.

Wed treatment attenuated renal morphological changes in UUO mice. a Histological examination of renal injury by H&E staining for sham-operated, UUO and Wed-treated UUO mice, Magnification, × 100, Bar = 1 mm. b Assessment of interstitial fibrosis by Masson staining, Magnification, × 200, Bar = 500 μm. c Representative image of Sirius Red staining under standard illumination, Magnification, × 100, Bar = 1 mm. d Representative image of Sirius Red staining under polarized illumination, Magnification, × 100, Bar = 1 mm. e Immunohistochemistry of α-SMA. Magnification, × 200, Bar = 500 μm. f Quantitative analysis of tubular injury score. g Quantitative analysis of fibrosis area. h Quantitative analysis of α-SMA positive area. ^*P<0.05 versus sham. ^#P<0.05 versus UUO