Fig. 6.

Caspase-11 knockdown reduced Ang II-induced inflammatory activation in renal TECs. Cells were transfected with caspase-11 siRNA (50 nmol/L) or NC siRNA (50 nmol/L) for 48 h and then treated with Ang II (1 μmol/L) for 24 h. a Pro-caspase-11 and caspase-11 were detected by western blotting. b Quantification of pro-caspase-11 and caspase-11. c Pro-caspase-1, pro-IL-1β, caspase-1, and IL-1β were detected by western blotting. d Quantification of pro-caspase-1, caspase-1, pro-IL-1β, and IL-1β. e IL-1β concentrations in the supernatants were measured by ELISA. Data are presented as the mean ± SEM (n = 6). ^*P < 0.05 versus NC siRNA. ^**P < 0.01 versus NC siRNA. ^#P < 0.05 versus Ang II + NC siRNA