Fig. 7.

Caspase-11 formed a protein complex with caspase-1. a NRK-52E cells were transfected with caspase-11 siRNA (50 nmol/L) or NC siRNA (50 nmol/L) for 48 h and then treated with Ang II (1 μmol/L) for 24 h. Levels of intracellular caspase-11 and caspase-1 were examined via immunofluorescence using anti-caspase-11 (red), anti-caspase-1 (green), and DAPI (blue). Magnification: × 200, Bar = 45 μm. b NRK-52E cells were treated with Ang II for 12 h. Cell lysates were immunoprecipitated with anti-caspase-1 antibody and analyzed by immunoblotting with anti-caspase-11 antibody