Fig. 4.

The role of PARP1 in the subcellular localization of HMGB1. NRCMs were infected with Ad-PARP1 or Ad-GFP (empty vector) for 48 h. In addition, another group of NRCMs were transfected with si-PARP1 or negative control (NC) for 72 h. a, b The nuclear and cytoplasmic protein were extracted respectively from NRCMs, and were observed by Western blot analysis. c, d Western blot analysis was conducted to determine the intracellular proteins of HMGB1 and PARP1. The results were normalized to those of α-Tubulin/Lamin B1 and were presented as the means ± SEM. *P < 0.05 vs Ad-GFP group or NC group, n = 5. e, f Immunofluorescence (IF) assay was performed to detect the subcellular distribution of HMGB1. Representative images of five independent experiments are shown. Con means control, NC means negative control