Fig. 3.

The anti-hTFPI-neutralizing antibody abolishes the protective effect of the hTFPI fusion protein expressed by CD31-TFPI-Tg mice. Total cell numbers (a), neutrophil numbers (b), and total protein (l) in BALF after intratracheal instillation of LPS for 24 h. With quantitative real-time PCR, the gene expression levels of KC (c), MIP-2 (e), IL-6 (g), TNF-α (i), IL-1β (j), and IL-10 (k) were detected. With Quantikine ELISA analysis, the protein expression levels of KC (d), MIP-2 (f), and IL-6 (h) were measured. Representative light photomicrographs (100 × ) of H&E staining of lung tissue (m) and histology score (n). Scale bars = 200 μm. Under the heading of “10⁴ cells/mL”, a value of 1 designates 10 000 cells/mL. n = 6–10 for each group. ^*P < 0.05; ^**P < 0.01; ^***P < 0.001; ns no significance. Data are presented as mean ± SEM of three separate experiments. CON control, LPS lipopolysaccharide, ALI acute lung injury, BALF bronchoalveolar lavage fluid, WT wild type