Fig. 5.

The expression level of CCL2 decreases in lung tissues after LPS-induced ALI in CD31-TFPI-Tg and CD31-Hir-Tg mice, either those engrafted with WT BM or those non-engrafted. Quantitative real-time PCR and ELISA analysis respectively detected the expression level of CCL2 in lung tissues in ALI model of CD31-TFPI-Tg and CD31-Hir-Tg mice (a, b), CD31-TFPI-Tg mice receiving anti-hTFPI-neutralizing antibody (c, d), and WT/CD31-TFPI-Tg and WT/CD31-Hir-Tg mice (e, f). n = 6–10 for each group. ^*P < 0.05; ^**P < 0.01; ^***P < 0.001; ns no significance. Data are presented as mean ± SEM of three separate experiments. CCL2 chemokine (C-C motif) ligand 2, CON control, LPS lipopolysaccharide, ALI acute lung injury, WT wild type