Fig. 3.

HOTAIR regulates miR-520b as a competing endogenous RNA. a The binding region between HOTAIR and miR-520b is highlighted in red. The MUT sequence of HOTAIR is highlighted in yellow. b The luciferase activity decreased significantly in the WT HOTAIR transfection group compared with that in the control, and the luciferase activity for the MUT HOTAIR group did not change significantly compared with that in the control (*P < 0.05 compared with the control). c After the overexpression of HOTAIR in DU145 and PC3 cells, the total protein was incubated with an IgG or AGO2 antibody, and the HOTAIR and miR-520b expression levels were significantly higher in the AGO2 group than they were in the IgG group (*P < 0.05 compared with the IgG group)