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. 2019 Apr 26;40(9):1228–1236. doi: 10.1038/s41401-019-0234-8

Fig. 6.

Fig. 6

The HOTAIR/miR-520b/FGFR1 axis plays an important role in the effects of bufalin on PCa cells. a The viability ratio of DU145 and PC3 cells in the HOTAIR overexpression group was significantly higher than that in the NC group after bufalin treatment (*P < 0.05 compared with the blank group, ##P < 0.05 compared with the bufalin + NC group). b The wound healing assay revealed that the overexpression of HOTAIR reversed the suppressive effect of bufalin on the migration of PCa cells. c The Transwell assay demonstrated that HOTAIR significantly promoted PCa cell invasion. d Comparisons of the migration rates and relative invasion rates between the NC and HOTAIR groups are shown (*P < 0.05). e Bufalin upregulated the relative level of miR-520b in PCa cells, and this effect was reversed by the overexpression of HOTAIR (*P < 0.05 compared with the blank group, ##P < 0.05 compared with the bufalin + NC group). f, g The overexpression of HOTAIR counteracted the downregulation effects of bufalin on expression of FGFR1 at both the mRNA (f) and protein levels (g) (*P < 0.05 compared with the blank group, ##P < 0.05 compared with the bufalin + NC group)