Fig. 6.

Nrf2 mediates the anti-inflammatory effects of MLB. a Western blot showing the effects of MLB on Nrf2 phosphorylation and HO1 expression (n = 5). b Western blot showing the effects of siRNA-mediated Nrf2 knockdown on stabilization of IκBα by MLB (n = 5). c siRNA-mediated Nrf2 knockdown abrogated the reduction in ICAM1 mRNA induced by MLB pretreatment (n = 6). HMEC-1 cells were transfected with Nrf2 siRNA for 48 h, pretreated with MLB for 12 h, and then stimulated with LPS. Data are expressed as means ± SEM (*P < 0.05, **P < 0.01, ***P < 0.001)