Fig. 8.

PI3K/Akt is involved in the anti-inflammatory effects of MLB. a MLB promoted phosphorylation of Akt in a concentration-dependent manner. b LY294002 reversed MLB-mediated Nrf2 activation. c MLB-induced stabilization of IκBα was attenuated by LY294002. d LY294002 prevented the inhibitory effects of MLB on ICAM1 mRNA levels. HMEC-1 cells were incubated with 100 μM MLB for 12 h in the presence or absence of LY294002 (10 μM) and then stimulated with LPS (1 μg/mL). Protein and total RNA samples were extracted 0.5 and 2 h after LPS stimulation, respectively, for detection of IκBα protein and evaluation of ICAM1 mRNA level. The results are representative of five independent experiments. Data are expressed as means ± SEM (*P < 0.05, **P < 0.01, ***P < 0.001)