Fig. 1.

NTZ promoted Aβ clearance by stimulating autophagy. a Structure of NTZ. b–e BV2 cells or astrocytes were treated with different concentrations of NTZ (20, 10, 5, or 0 μM) for 24 h, followed by the addition of 2 μg/mL soluble Aβ₄₀/Aβ₄₂ and incubation for 3 h. Aβ₄₀/Aβ₄₂ level was detected by ELISA assay (t test, ^*P < 0.01; ^**P < 0.05; ^***P < 0.001 vs DMSO group). f Aβ₄₀ level in the supernatant was detected by ELISA assay. g Immunofluorescence images of BV2 cells treated with NTZ and 2 μg/mL soluble Aβ₄₀. h Quantitative results of Figures g. i BV2 cells were treated with DMSO, NTZ (20 μM), CQ (20 μM) and NTZ (20 μM) in combination with CQ (20 μM), and 2 μg/mL soluble Aβ₄₀ was added into the cells and incubated for 3 h. Aβ₄₀ level was detected by ELISA assay. (one-way ANOVA, ^**P < 0.01 vs DMSO group; ^#P < 0.05 vs NTZ combined with CQ; ^&P < 0.01 vs DMSO group). All data were obtained from three independent experiments and are presented as the mean ± SEM