Fig. 4.

NTZ reduced tau hyperphosphorylation by inhibiting GSK3β activity. a–l SH-SY5Y (a, e, i) or neuronal (c, g, k) cells were cultured with different concentrations of NTZ (20, 10, 5 or 0 μM) for 24 h, and phosphorylation levels of PI3K, AKT, GSK3β and Tau were detected by Western blot. b, f, j Quantitative results of (a, e, and i). d, h, l Quantitative results of Figures c, g and k. GAPDH was used as a loading control in Western blot assays. (one-way ANOVA. n = 3. ^*P < 0.05; ^**P < 0.01; ^***P < 0.001 vs DMSO group). All data were obtained from three independent experiments and are presented as the mean ± SEM