Fig. 3.

Minocycline inhibits NF-κB activation during OGD/R-induced I/R injury in H9c2 cardiomyocytes. a NF-кB p65 protein levels in nuclear and cytosolic extracts were assayed by Western blot, and densitometry measurements of the nuclear p65 protein are normalized to Lamin B or GAPDH. b Representative figures of NF-κB p65 nuclear translocation imaged by laser confocal immunofluorescent microscopy. Scale bar = 20 µm. Quantitative data are presented as the mean ± SEM. The results are representative of three independent experiments; ^*P < 0.05, ^**P < 0.01 versus CTL; ^#P < 0.05, ^##P < 0.01 versus the OGD/R model