Fig. 4.

Minocycline induces MCPIP1 expression during I/R-induced injury in vivo and OGD/R-induced I/R injury in vitro. a MCPIP1 protein levels in ischemic myocardial tissue were measured by Western blot at I (1 h)/R (3 h), I (1 h)/R (24 h), and I (1 h)/R (48 h). b MCPIP1 protein levels with and without minocycline treatment were measured by Western blot after I (1 h)/R (48). c MCPIP1 protein levels in H9c2 were measured by Western blot at different oxygen glucose deprivation (OGD)-reoxygenation times (OGD6 h/R3, 6, 12 h). d MCPIP1 protein levels with minocycline treatment in H9c2 cells subjected to OGD/R were measured in a concentration-dependent manner by Western blot. Quantitative data are presented as the mean ± SEM. n = 6 rats per group or 6 examples of cells; ^*P < 0.05, ^**P < 0.01 versus Sham or CTL; ^#P < 0.05, ^##P < 0.01 versus I/R model or OGD/R model. MCPIP1, monocyte chemotactic protein-induced protein-1