Fig. 6.

MCPIP1 knockdown promotes the expression of MCP-1 and IL-6 and reduces cell viability during OGD/R-induced I/R injury in vitro. The relative mRNA levels of pro-inflammatory cytokines a MCP-1, b IL-6, and c TNF-α were measured by qPCR after knockdown of MCPIP1-1 in H9c2 cells. d Representative images of H9c2 cell morphology were obtained under a light microscope. e The viability of H9c2 cells was determined by MTS assay after siMCPIP1-1 transfection. Scale bar = 100 µm. Quantitative data are presented as the mean ± SEM. n = 6; ^*P < 0.05, ^**P < 0.01 versus CTL; ^#P < 0.05, ^##P < 0.01 versus OGD/R model; ^$P < 0.05, ^$$P < 0.01 versus siControl. siControl, negative control siRNA; siMCPIP, MCPIP1 siRNA