Fig. 7.

Minocycline protects H9c2 cardiomyocytes against OGD/R-induced I/R injury in H9c2 cardiomyocytes via MCPIP1. a The cell viability was determined by MTS assay, and the relative mRNA levels of the pro-inflammatory cytokines b MCP-1, and c IL-6 were measured by qPCR in H9c2 cells subjected to OGD/R injury pretreated with minocycline after knockdown of MCPIP1 by RNAi. Quantitative data are presented as the mean ± SEM. n = 6; ^*P < 0.05, ^**P < 0.01 versus CTL; ^#P < 0.05, ^##P < 0.01 versus OGD/R model; ^$P < 0.05, ^$$P < 0.01 versus siControl + OGD/R + MINO. siControl, negative control siRNA; siMCPIP, MCPIP1 siRNA