Table 1.
Gene | Sequence |
---|---|
MCPIP1 | F: 5′-CCAGGACTCCAGTCAAGGACAA-3′; |
R: 5′-TGGCTCAGCCTCTAGGGACA-3′; | |
TNF-α | F: 5′-GGCGTGTTCATCCGTTCTC-3′; |
R: 5′-CTTCAGCGTCTCGTGTGTTTCT-3′; | |
IL-6 | F: 5′-ATTGTATGAACAGCGATGATGCAC-3′; |
R: 5′-CCAGGTAGAAACGGAACTCCAGA-3′; | |
MCP-1 | F: 5′-CTATGCAGGTCTCTGTCACGCTTC-3′; |
R: 5′-CAGCCGACTCATTGGGATCA-3′; | |
GAPDH | F: 5′-ACTCACTCAAGATTGTCAGCAATG-3′; |
R: 5′-TCATGAGCCCTTCCACAATGC-3′ | |
siRNA oligonucleotides | |
MCPIP1-1 | 5′-UGAAAUGGCCAGUACCGAUGGCUUG-3′ |
MCPIP1-2 | 5′-UUCUUACGCAGGAAGUUGUCCAGGC-3′ |
MCPIP1-3 | 5′-AAGAAAGGAUCUCAGCGGCCAGCUG-3′ |
Control | Cat#12935400 |
F forward sequence, R reverse sequence