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. 2018 Nov 26;40(7):957–970. doi: 10.1038/s41401-018-0184-6

Table 2.

Human primer sequences for qRT-PCR

Gene Forward primer sequence Reverse primer sequence
MRE11 meiotic recombination 11 MRE11 5′-CTCAGCAGCAACCAACAAAG-3′ 5′GTGTCTCTTCCTCTTTGAGACC-3′
RAD50 homolog RAD50 5′-GAATCTAGGCTTCTCAATCAGG-3′ 5′-CTCTAGCTCGGATATGTTCTTG-3′
Nibrin NBS1 5′-CCACCTCCAAAGACAACTG-3′ 5′-AGGACGGCAGGAAAGAA-3′
CHECK1 checkpoint homolog CHECK1 5′-TCTGTTGGATGAAAGGGATAAC-3′ 5′-TCAACAAACGCTCACGATTA-3′
CHECK2 checkpoint homolog CHECK2 5′-GTGTCCACTCAGGAACTCTATTC-3′ 5′CATCCTGAAGGGCCCATAATC-3′
Breast cancer 1, early onset BRCA1 5′-CAAGAAGAGCAAAGCATGGA-3′ 5′-GAGGATAGCCCTGAGCAGTC-3′
Breast cancer 2, early onset BRCA2 5′-TTTGCGTTGAGGAACTTGTG-3′ 5′-AGGTGGCCCTACCTCAAAAT-3′
RAD51 homolog RAD51 5′-AAAGGAAGAGGGGAAACCAG-3′ 5′-ATCTCCCACTCCATCTGCAT-3′
RAD17 homolog RAD17 5′-GTGGTTTAAGGAGCGGTCAG-3′ 5′-TTTGCATCCTGAATTAGTCCTTC-3′
MutS homolog 2 MSH2 5′-AAGAAGTGCTATCTGGAAAGAG-3′ 5′-ACATTTCAGTAAAGGGCATTTG-3′
Exonuclease 1 EXO1 5′-AGGGAAAGCAACTTCTTCG-3′ 5′-GCCATGGCATGTGTGATA-3′
Vitamin D receptor VDR 5′- ATGCCATCTGCATCGTCTC-3′ 5′-GCACCGCACAGGCTGTCCTA-3′
18 S Ribosomal RNA 18 S rRNA 5′-CGAACGTCTGCCCTATCAACTT-3′ 5′-ACCCGTGGTCACCATGGTA-3′