Fig. 6.

Rab7 receptor antagonist inhibits the co-localization of cathepsin B and Rab7. a Representative images of cathepsin B, GFAP, and Hoechst staining in astrocytes after OGD for 6 h and reoxygenaration for 24 h (cathepsin B: green; GFAP: red; Hoechst: blue).Quantification of green fluorescence intensity of cathepsin B immunostaining. Mander’s overlap coefficients demonstrated the co-localization between cathepsin B and GFAP. Mean ± SD. n = 6. ^##P < 0.01 vs. non-OGD-Re24 h group; ^*P < 0.05, ^**P < 0.01 vs. OGD6 h-Re24 h group. b Representative images of cathepsin B, Rab7, and Hoechst staining in astrocytes after OGD for 6 h and reoxygenaration for 24 h (cathepsin B: green; Rab7: red; Hoechst: blue). Mander’s overlap coefficients and Pearson’s overlap coefficients demonstrated the co-localization between cathepsin B and Rab7. Mean ± SD. n = 6. ^##P < 0.01 vs non-OGD-Re24 h group; ^**P < 0.01 vs. OGD6h-Re24h group. Statistical analysis was carried out with a one-way ANOVA followed by a post hoc Tukey test