Table 1.
Primer sequences for genes used in RT-PCR assay
| Primer | S or AS | Sequence (5′–3′) |
|---|---|---|
| Tnf-α | S | AAGCCTGTAGCCCACGTCGTA |
| AS | GGCACCACTAGTTGGTTGTCTTTG | |
| Il-6 | S | TGGGGCTCTTCAAAAGCTCC |
| AS | AGGAACTATCACCGGATCTTCAA | |
| Il-1β | S | GGTGTGTGACGTTCCCATTAGAC |
| AS | CATGGAGAATATCACTTGTTGGTTGA | |
| Activin | S | GGGCCTTTTGATGCTGTCG |
| AS | TGGCAGAATGGTCTCTTGCAG | |
| Follistatin | S | CAAGCCCAGAAGGTCCTACAG |
| AS | GACTTGTGCATGTCTCCCCAG | |
| Collagen-1 | S | GCCCAGAGATAGAGTGACCTG |
| AS | CGCACTTCTCGAAAGTTGCTG | |
| Collagen-3 | S | ACATGCTCAACGTGAAGGG |
| AS | TGCCAACTTGGAAGCAGTCAT | |
| Mmp2 | S | TGGGGGAGATTCTCACTTTG |
| AS | CCATCAGCGTTCCCATACTT | |
| Mmp9 | S | CCACCACAGCCAACTATGACCAG |
| AS | AAGACGAAGGGGAAGACGCACA | |
| Mmp13 | S | AGGTTGAGGCTGAGCTCTTT |
| AS | AAGGTCACGGGATGGATGTT | |
| Timp1 | S | CAGTGTTTCCCTGTTTATCTATCCC |
| AS | AGTGACGGCTCTGGTAGTCC | |
| Timp2 | S | TGGACGTTGGAGGAAAGAAGGAG |
| AS | TTCTGGGTGATGCTAAGCGTGTC | |
| Timp3 | S | ACTACTTGCCTTGTTTTGTGA |
| AS | GCTGATGCTCTTGTCTGG | |
| Collagen-Iα1 | S | GCTCCTCTTAGGGGCCACT |
| AS | CCACGTCTCACCATTGGGG | |
| Collagen-Iα2 | S | GTAACTTCGTGCCTAGCAACA |
| AS | CCTTTGTCAGAATACTGAGCAGC | |
| β-Actin | S | GGCTGTATTCCCCTCCATCG |
| AS | CCAGTTGGTAACAATGCCATGT |
S sense primer, AS antisense primer