Fig. 5.

c-FLIP is decreased in kidneys of I/R rats and the decrease in c-FLIP is responsible for FasL-induced cell apoptosis. a Western blotting was performed to visualize the protein levels of c-FLIP in kidneys of rats (six rats per group). b The protein levels of c-FLIP were assayed by Western blotting in NRK-52E cells with or without 6 h hypoxia and 3 or 6 h reoxygenation treatment (three experiments per group). c NRK-52E cells were transfected with c-FLIP overexpression plasmid for 24 h. The protein levels of c-FLIP were assayed by Western blotting (three experiments per group). d The protein levels of cleaved caspase-8 and cleaved caspase-3 were assayed by Western blotting in FasL (0.125 μg/mL)-treated NRK-52E cells with or without pretreatment with c-FLIP expression plasmid (three experiments per group). e The protein levels of cleaved caspase-8 and cleaved caspase-3 were assayed by Western blotting in FasL (0.125 μg/mL)-treated NRK-52E cells with or without pretreatment with c-Myc inhibitor 10058-F4 (three experiments per group). Data are presented as the mean ± SEM. ^*P < 0.05 versus control, ^**P < 0.01 versus control or pcDNA 3.0. ^#P < 0.05 versus FasL. PcDNA 3.0, empty plasmid vector; c-FLIP plasmid, an plasmid harboring c-FLIP gene