Fig. 6.

c-Myc binds to the promoter region of c-FLIP gene. a c-Myc binding sites in c-FLIP promoter predicted by JASPAR 2018. b Schematic diagram of the binding site of c-Myc in c-FLIP promoter. Red words indicate c-Myc binding motif. c c-Myc bound to the proximal promoter of the c-FLIP gene as determined by chromatin immunoprecipitation assay. Cross-linked nuclear extracts of NRK-52E cells transduced with c-Myc virus vectors (Ad-c-Myc) were immunoprecipitated using either an anti-c-Myc antibody or control IgG. The regions of the c-Myc binding sites were quantified and normalized to IgG pulldown. d Luciferase expression analysis using wild-type (c-FLIP WT) and mutant c-FLIP promoter regions (c-FLIP mut) in NRK-52E cells 1 day after transfection with empty vector (Ad-Control) or Ad-c-Myc. Mutant c-FLIP promoter was constructed with a 10-bp deletion of the binding site. Data are presented as the mean ± SEM. ^**P < 0.01 versus IgG. ^***P < 0.001 versus Ad-Control