Fig. 1.

Osthole reduced TMX-induced liver injury. a, b Mice were intraperitoneally injected with osthole (100 mg/kg). After 24 h, TMX (90 mg/kg) was administered to mice. Mice were sacrificed at the indicated times after the TMX injection. Serum ALT and AST activities were determined. Data are reported as the mean ± SEM, n = 4–5 mice per group. *P < 0.05, **P < 0.01 compared to 0 h, ^#P < 0.05, ^##P < 0.01 compared to TMX, ^§P < 0.05 compared to 5 h, ^¶¶P < 0.01 compared to 8 h. c, d Dose-dependent responses of serum ALT and AST activities in mice to osthole 8 h after the TMX treatment. Data are presented as the mean ± SEM, n = 4–5 mice per group. *P < 0.05, **P < 0.01 compared to the control, ^#P < 0.05, ^##P < 0.01 compared to TMX. e Representative images of H&E-stained liver sections (×100 magnification)