Fig. 2.

Osthole reduced TMX-induced oxidative stress. Mice were intraperitoneally injected with osthole (100 mg/kg). After 24 h, TMX (90 mg/kg) was administered to mice. Mice were sacrificed 8 h after the TMX injection. Serum H₂O₂ (a), MDA (b), and GSH levels (c) and the GSSG-to-GSH ratio (d) were determined. Hepatic levels of H₂O₂ (e), GSH (f), ROS (detected using DHE staining) (g), and lipid peroxides (h) were examined. Data are presented as the mean ± SEM, n = 4–5 mice per group. **P < 0.01 compared to the control, ^#P < 0.05, ^##P < 0.01 compared to TMX