Fig. 3.

Osthole significantly increased the expression of antioxidant genes (GPX1, SOD2, GCL-c, and G6pdh), but suppressed the expression of pro-oxidant genes (NOX2 and ACOX). Mice were intraperitoneally injected with osthole (100 mg/kg). After 24 h, TMX (90 mg/kg) was administered to mice. Mice were sacrificed 8 h after the TMX injection, and the mRNA levels in the liver were quantified using real-time RT-PCR. Data are presented as the mean ± SEM, n = 4–5 mice per group. *P < 0.05, **P < 0.01 compared to the control, ^#P < 0.05 compared to TMX