Fig. 4.

Osthole reduced the TMX-induced inflammatory response. Mice were intraperitoneally injected with osthole (100 mg/kg). After 24 h, TMX (90 mg/kg) was administered to mice. Mice were sacrificed 8 h after the TMX injections. a–d The levels of inflammation-related mRNAs in the liver were quantified using real-time RT-PCR. e, f Hepatic IL-1β and IL-6 levels were detected using ELISAs. Data are presented as the mean ± SEM, n = 4–5 mice per group. *P < 0.05, **P < 0.01 compared to the control, ^#P < 0.05, ^##P < 0.01 compared to TMX