Fig. 7.

Osthole attenuated TMX-induced liver injury by inhibiting P38 phosphorylation. a Mice were intraperitoneally injected with osthole (100 mg/kg). After 24 h, TMX (90 mg/kg) was administered to mice. Mice were sacrificed 8 h after the TMX injections. Western blot analyses of the liver homogenates were performed. b–d Quantitation of the data shown in a. e–i Mice were injected with the p38 inhibitor SB203580 (2 mg/kg) 1 h before the TMX injection. Serum ALT and AST activities were measured 8 h after the TMX injection (e, f). Hepatic H₂O₂ (g), MDA (h), and GSH (i) levels were measured. Data are reported as the mean ± SEM, n = 4–6 mice per group. *P < 0.05, **P < 0.01 compared to the control, ^#P < 0.05, ^##P < 0.01 compared to TMX