Fig. 6.

Hispidulin exhibits anti-tumor activity in vivo through the activation of the ERS signaling pathway. a Measurement of the body weight and tumor volume every 3 days in a HCC xenograft mice model after treatment with different dosages of hispidulin (25 and 50 mg/kg/day). b TUNEL assay analysis of apoptotic cell cryostat sections (4 μm sections) of HCC xenograft tumors after treatment with hispidulin. c Immunohistochemistry staining was performed on the cryostat sections (4 μm sections) of HCC xenograft tumors to detect the expression of CHOP, Bcl-2, cleaved caspase-3, and Ki-67 after treatment with hispidulin. d The protein expression of CHOP, Bcl-2, GRP78, cleaved caspase-3, and cleaved caspase-12 in tumor tissues was detected by immunoblot analysis. Data are presented as the mean ± SD, n = 8. *P < 0.05; **P < 0.01