Fig. 6.

The DPP4 inhibitor sitagliptin significantly reverses HPH. A rat model of HPH was generated as described previously (n = 8/group). The experimental groups were treated with the indicated doses of sitagliptin. a Western blotting of lung tissue with the indicated antibodies. All phospho-protein levels were measured by densitometry and normalized to that of total protein. The (b) RVP and (c) RV/BW and RV/(LV+S) ratios were calculated. The data are expressed as the means ± standard errors of the mean (n = 8). *P < 0.05, **P < 0.01, ***P < 0.001. d HE staining of paraffin-fixed lung sections for the morphological analysis of pulmonary arteries. e Immunofluorescence co-staining of Ki-67 and α-SMA in small pulmonary arteries (40×, n = 8)