Figure 1.

OC mediates autophagosome maturation by engaging STX17 and blocks autophagosome-lysosome fusion. a OC mediated autophagosome maturation by engaging STX17. HeLa cells stably expressing GFP-LC3 were treated with OC (15 μM) or CQ (50 μM) for 8 h and then stained with anti-STX17 antibody. The cells were imaged by confocal microscopy. b Quantification of colocalization of LC3 with STX17 using Pearson’s correlation coefficient (Student t-test, ^**P < 0.01 compared to DMSO). c OC blocked autophagosome-lysosome fusion. The tfLC3B-L929 cells were treated with OC (15 μM), CQ (50 μM), or PP242 (1 μM) for 8 h. The cells were imaged by confocal microscopy. d OC induced the accumulation of P62 and LC3 II in HeLa cells. HeLa cells were treated with OC at the indicated concentration and time. The cell lysates were analyzed by Western blotting using antibodies targeting P62, LC3 and β-actin. e OC inhibited autophagic flux. HeLa cells were treated with OC (15 μM) or PP242 (1 μM) for 24 h in the presence or absence of CQ (50 μM). The cell lysates were analyzed by Western blotting using antibodies targeting P62, LC3 and β-actin