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. 2018 Oct 17;40(7):929–937. doi: 10.1038/s41401-018-0167-7

Figure 2.

Figure 2

OC induces TFEB nuclear translocation. a OC enhanced TFEB nuclear translocation. HeLa cells were treated with OC (15 μM) or Torin1 (1 μM) for 8 h and then stained with anti-TFEB antibody for immunofluorescence analysis. b, c HeLa cells were treated with OC (15 μM), Torin1 (1 μM) or PP242 (1 μM) for 8 h. Nuclear fractions and cytoplasm lysates were prepared and then analyzed by Western blot using anti-TFEB, anti-LaminA + C and anti-GAPDH antibodies. *P < 0.05, treatment with OC, Torin1 and PP242 compared to DMSO. d OC mediated the TFEB mobility-shift. HeLa or MEF cells were treated with OC (15 μM) or PP242 (1 μM) for 8 h, and endogenous TFEB was analyzed by Western blotting using anti-TFEB and anti-actin antibodies. e Cotreatment with OC and okadaic acid (OA) inhibited TFEB dephosphorylation. HeLa cells were treated with 15 μM OC alone or in combination with OA for 8 h and subjected to Western blot analysis. f The combination of OC and OA inhibited TFEB nuclear translocation. HeLa cells were treated with 15 μM OC alone or in combination with 100 nM OA for 8 h and then subjected to Western blot analysis