Figure 5.

The combination of OC and hydroxycitrate inhibits growth of HeLa cells. a, b HeLa cells were treated with OC (5 or 7.5 μM) alone or in combination with HC (15, 20 or 25 mM) for 24 h and analyzed by flow cytometry. ^*P < 0.05, ^**P < 0.01, compared to the related groups linked by lines. c HeLa cells were treated with OC (5 or 7.5 μM) alone or in combination with HC (15, 20, or 25 mM) for 24 h, and the cell lysates were immunoblotted using anti-PARP, anti-caspase 9 and anti-GAPDH antibodies. d The model represents the mechanism by which OC regulates autophagy