Figure 3.

Effects of inhibitors of nitric oxide synthase (NOS) and Ca²⁺-activated Cl⁻ channel (Ano1) on colonic migrating motor complexes (CMMCs) in the colonic muscles of control (Con) and partial colon obstruction (PCO) mice. (A, B) The inhibitory effects of NG-Nitro-l-argi-nine methyl ester hydrochloride (L-NAME; 100 μM) on CMMCs in the proximal and distal colons of both control and PCO mice. (C, D) The area under the curve (AUC) were normalized to the control (before the application of L-NAME) (n = 5; *P < 0.05, L-NAME vs control; ^#P < 0.05, PCO vs control mice). (E, F) Response of CMMCs to 5-Nitro-2-(3-phenylpropylamino) benzoic acid (NPPB; 5 μM) in the proximal and distal colons in both control and PCO mice. (G, H) Summary of the response of CMMCs to NPPB in colonic muscles of control and PCO mice. The data were normalized to the control value (before the application of NPPB) (n = 5; *P < 0.05, NPPB vs control; ^#P < 0.05, PCO vs control mice).