Fig. 3.

eEF-2K is involved in doxorubicin-induced activation of autophagy. a SK-MEL-5, SK-MEL-28, or A-375 cells were treated with a series of doxorubicin (Dox) concentrations for 24 h, and the levels phospho-eEF2 (T56) were then measured by Western blot. β-actin was used as the loading control. b SK-MEL-5, SK-MEL-28, or A-375 cells were treated with 1 μmol/L doxorubicin with or without eEF-2K silencing. The LC3 and phospho-eEF2 (T56) levels were measured by Western blot. β-actin was used as the loading control